Human Complement component C8 alpha chain ELISA Kit | C8A elisa kit

Human Complement component C8 alpha chain ELISA Kit

Cat. Num. AAA765212

• Reactivity: Human
• Synonyms: Complement component C8 alpha chain; Human Complement component C8 alpha chain ELISA Kit; C8A (Complement component C8 alpha chain); C8A elisa kit

• Reactivity: Human
• Sequence Length: 584

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 12.5-800ng/ml
• Sensitivity: 7.5ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C8A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human C8Aantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human C8A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human C8A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman C8Acan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557389
• NCBI GeneID: 731
• NCBI Accession #: NP_000553.1
• NCBI GenBank Nucleotide #: NM_000562.2
• Molecular Weight: 65,163 Da
• NCBI Official Full Name: complement component C8 alpha chain preproprotein
• NCBI Official Synonym Full Names: complement component 8, alpha polypeptide
• NCBI Official Symbol: C8A
• NCBI Protein Information: complement component C8 alpha chain
• UniProt Protein Name: Complement component C8 alpha chain
• Protein Family: Complement component
• UniProt Gene Name: C8A
• UniProt Entry Name: CO8A_HUMAN

NCBI Description

C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008]

Uniprot Description

C8A: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself. Defects in C8A are a cause of complement component 8 deficiency type 1 (C8D1). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: extracellular region; extracellular space; membrane; membrane attack complex

Molecular Function: complement binding; protein complex binding

Biological Process: complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis; immune response; innate immune response; regulation of complement activation

Disease: Complement Component 8 Deficiency, Type I