Mouse Growth Differentiation Factor 3 ELISA Kit | GDF3 elisa kit

Mouse Growth Differentiation Factor 3 ELISA Kit

Cat. Num. AAA765097

• Reactivity: Mouse
• Synonyms: Growth Differentiation Factor 3; Mouse Growth Differentiation Factor 3 ELISA Kit; GDF3/Vgr-2/GDF-3/growth differentiation factor 3/growth/differentiation factor 3/KFS3/MCOP7/MCOPCB6; GDF3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GDF11. No significant cross-reactivity or interference between GDF11 and analogues was observed.
• Sequence Length: 364

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: < 9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF11were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF11were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GDF11antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GDF11 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF11 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9652072
• Molecular Weight: 41,387 Da
• NCBI Official Full Name: growth differentiation factor 3
• UniProt Protein Name: Growth/differentiation factor 3
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF3
• UniProt Synonym Gene Names: GDF-3
• UniProt Entry Name: GDF3_HUMAN

Uniprot Description

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; protein kinase binding; transforming growth factor beta receptor binding

Biological Process: BMP signaling pathway; cell development; endoderm development; eye development; formation of anatomical boundary; growth; in utero embryonic development; mesoderm development; negative regulation of BMP signaling pathway; negative regulation of epidermal cell differentiation; negative regulation of myoblast differentiation; notochord development; regulation of apoptosis; regulation of cell fate commitment; regulation of MAPKKK cascade; response to dietary excess; skeletal development; somite rostral/caudal axis specification

Disease: Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated 7; Microphthalmia, Isolated, With Coloboma 6