Human Integrin alpha-L ELISA Kit | ITGAL elisa kit

Human Integrin alpha-L ELISA Kit

Cat. Num. AAA763838

• Gene Names: ITGAL; CD11A; LFA-1; LFA1A
• Reactivity: Human
• Synonyms: Integrin alpha-L; Human Integrin alpha-L ELISA Kit; ITGAL/Integrin alpha-L/Leukocyte function-associated molecule 1 alpha chain/CD11 antigen-like family member A/Leukocyte adhesion glycoprotein LFA-1 alpha chain/LFA-1A/CD11A/LFA-1/LFA1A; ITGAL elisa kit

• Reactivity: Human
• Sequence Length: 1170

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ITGAL elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ITGAL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ITGAL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ITGAL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ITGAL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 167466215
• NCBI GeneID: 3683
• NCBI Accession #: NP_002200.2
• NCBI GenBank Nucleotide #: NM_002209.2
• Molecular Weight: 119,224 Da
• NCBI Official Full Name: integrin alpha-L isoform a
• NCBI Official Synonym Full Names: integrin subunit alpha L
• NCBI Official Symbol: ITGAL
• NCBI Official Synonym Symbols: CD11A; LFA-1; LFA1A
• NCBI Protein Information: integrin alpha-L
• UniProt Protein Name: Integrin alpha-L
• Protein Family: Integrin
• UniProt Gene Name: ITGAL
• UniProt Synonym Gene Names: CD11A; LFA-1A
• UniProt Entry Name: ITAL_HUMAN

NCBI Description

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell surface; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; external side of plasma membrane; immunological synapse; integrin complex; intercellular junction; membrane; plasma membrane

Molecular Function: cell adhesion molecule binding; ICAM-3 receptor activity; metal ion binding; protein binding; protein complex binding; protein heterodimerization activity

Biological Process: activated T cell proliferation; blood coagulation; cell adhesion; cell motility; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; inflammatory response; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; positive regulation of calcium-mediated signaling; positive regulation of T cell proliferation; receptor clustering; regulation of immune response; signal transduction; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell