Rat Glucose Transporter 3 ELISA Kit | GLUT3 elisa kit

Rat Glucose Transporter 3 ELISA Kit

Cat. Num. AAA763454

• Gene Names: SLC2A3; GLUT3
• Reactivity: Rat
• Synonyms: Glucose Transporter 3; Rat Glucose Transporter 3 ELISA Kit; GLUT3/Slc2a3/Glut-3/Glut3/Solute carrier family 2; facilitated glucose transporter member 3/Glucose transporter type 3; brain/GLUT-3; GLUT3 elisa kit

• Reactivity: Rat
• Sequence Length: 496

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for GLUT3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat GLUT3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat GLUT3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat GLUT3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat GLUT3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 8927560
• NCBI GeneID: 6515
• Molecular Weight: 53,924 Da
• NCBI Official Full Name: glucose transporter 3
• NCBI Official Synonym Full Names: solute carrier family 2 member 3
• NCBI Official Symbol: SLC2A3
• NCBI Official Synonym Symbols: GLUT3
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 3
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 3
• Protein Family: Glucose transporter
• UniProt Gene Name: SLC2A3
• UniProt Synonym Gene Names: GLUT3; GLUT-3
• UniProt Entry Name: GTR3_HUMAN

Uniprot Description

GLUT3: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. It???s surface expression is induced by insulin. It is restricted in humans to brain, testis (in the spermatozoa), and skeletal muscle, mostly in triads of slow-twitch fibres. Detected only in the brain of mouse and rat. IHC demonstrates its present in neurons, mainly at the plasma membrane, and to a lesser extent on intracellular vesicles distinct from synaptic vesicles. Present in the a-granules of human platelets, where it is translocated to the cell surface in response to thrombin stimulation. Its surface expression is increased by insulin or insulin growth-factor in cultured L6 muscle cells.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: glucose binding; glucose transmembrane transporter activity

Biological Process: carbohydrate metabolic process; glucose transport; hexose transport; L-ascorbic acid metabolic process; transmembrane transport; vitamin metabolic process; water-soluble vitamin metabolic process