Human Neurturin ELISA Kit | NRTN elisa kit

Human Neurturin ELISA Kit

Cat. Num. AAA763262

• Gene Names: NRTN; NTN
• Reactivity: Human
• Synonyms: Neurturin; Human Neurturin ELISA Kit; NRTN/NTN; NRTN elisa kit

• Reactivity: Human
• Sequence Length: 197

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NRTN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NRTN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NRTN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NRTN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NRTN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4758826
• NCBI GeneID: 4902
• NCBI Accession #: NP_004549.1
• NCBI GenBank Nucleotide #: NM_004558.3
• Molecular Weight: 22,405 Da
• NCBI Official Full Name: neurturin preproprotein
• NCBI Official Synonym Full Names: neurturin
• NCBI Official Symbol: NRTN
• NCBI Official Synonym Symbols: NTN
• NCBI Protein Information: neurturin
• UniProt Protein Name: Neurturin
• Protein Family: Neurturin
• UniProt Gene Name: NRTN
• UniProt Entry Name: NRTN_HUMAN

NCBI Description

Neurturin is a member of the TGF-beta subfamily, TRN. This gene signals through RET and a GPI-linked coreceptor, and promotes survival of neuronal populations. A neurturin mutation has been described in a family with Hirschsprung Disease. [provided by RefSeq, Jul 2008]

Uniprot Description

NRTN: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Belongs to the TGF-beta family. GDNF subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: axon; extracellular region; intracellular

Molecular Function: growth factor activity; receptor binding

Biological Process: axon guidance; MAPKKK cascade; nerve development; nervous system development; neural crest cell migration; neurite development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Hirschsprung Disease, Susceptibility To, 1