Mouse Galectin 9 ELISA Kit | GAL9 elisa kit

Mouse Galectin 9 ELISA Kit

Cat. Num. AAA763012

• Gene Names: LGALS9; HUAT; LGALS9A
• Reactivity: Mouse
• Synonyms: Galectin 9; Mouse Galectin 9 ELISA Kit; Galectin-9/GAL9/Galectin 9/Ecalectin/LGALS9/ecalectin/Ecalectin/Gal-9/gal-9/HUAT; GAL9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GAL9. No significant cross-reactivity or interference between GAL9 and analogues was observed.
• Sequence Length: 323

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.813-500pg/ml
• Sensitivity: 4.688pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAL9 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 102469694
• NCBI GeneID: 3965
• NCBI Accession #: NP_002299.2
• NCBI GenBank Nucleotide #: NM_002308.3
• Molecular Weight: 38,321 Da
• NCBI Official Full Name: galectin-9 isoform short
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 9
• NCBI Official Symbol: LGALS9
• NCBI Official Synonym Symbols: HUAT; LGALS9A
• NCBI Protein Information: galectin-9
• UniProt Protein Name: Galectin-9
• Protein Family: Gallinacin
• UniProt Gene Name: LGALS9
• UniProt Synonym Gene Names: Gal-9
• UniProt Entry Name: LEG9_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm; extracellular space; intracellular; nucleus

Molecular Function: carbohydrate binding; disaccharide binding; enzyme binding; galactose binding; signal transducer activity

Biological Process: activation of NF-kappaB transcription factor; chemotaxis; female pregnancy; inflammatory response; maternal process involved in pregnancy; natural killer cell tolerance induction; negative regulation of activated T cell proliferation; negative regulation of chemokine production; negative regulation of interferon-gamma production; negative regulation of mast cell degranulation; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of tumor necrosis factor production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation during immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-4 production; positive regulation of NF-kappaB import into nucleus; positive regulation of virion penetration into host cell; regulation of interleukin-4 production; regulation of interleukin-5 production; response to lipopolysaccharide; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway