Human Necdin ELISA Kit | NDN elisa kit

Human Necdin ELISA Kit

Cat. Num. AAA762895

• Gene Names: NDN; PWCR; HsT16328
• Reactivity: Human
• Synonyms: Necdin; Human Necdin ELISA Kit; NDN/Necdin; NDN elisa kit

• Reactivity: Human
• Sequence Length: 321

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NDN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Necdin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Necdin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Necdin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Necdin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505349
• NCBI GeneID: 4692
• NCBI Accession #: NP_002478.1
• NCBI GenBank Nucleotide #: NM_002487.2
• Molecular Weight: 36,086 Da
• NCBI Official Full Name: necdin
• NCBI Official Synonym Full Names: necdin, MAGE family member
• NCBI Official Symbol: NDN
• NCBI Official Synonym Symbols: PWCR; HsT16328
• NCBI Protein Information: necdin
• UniProt Protein Name: Necdin
• Protein Family: Necdin
• UniProt Gene Name: NDN
• UniProt Entry Name: NECD_HUMAN

NCBI Description

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008]

Uniprot Description

NDN: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q11.2-q12

Cellular Component: cell projection; centrosome; cytosol; nucleus; perikaryon

Molecular Function: gamma-tubulin binding

Biological Process: axon extension; axonal fasciculation; central nervous system development; glial cell migration; multicellular organismal homeostasis; negative regulation of cell proliferation; nerve growth factor receptor signaling pathway; nervous system development; neuron migration; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; regulation of growth; respiratory system process; sensory perception of pain; transcription from RNA polymerase II promoter

Disease: Prader-willi Syndrome