Mouse Carbonic anhydrase 9 ELISA Kit | CA9 elisa kit

Mouse Carbonic anhydrase 9 ELISA Kit

Cat. Num. AAA762796

• Gene Names: CA9; MN; CAIX
• Reactivity: Mouse
• Synonyms: Carbonic anhydrase 9; Mouse Carbonic anhydrase 9 ELISA Kit; CA9/ (Carbonic anhydrase 9)/CAIX/CA-IX/MN/Carbonic Dehydratase; CA9 elisa kit

• Reactivity: Mouse
• Sequence Length: 459

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CA9 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse CA9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse CA9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CA9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse CA9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 169636420
• NCBI GeneID: 768
• NCBI Accession #: NP_001207.2
• NCBI GenBank Nucleotide #: NM_001216.2
• Molecular Weight: 49,698 Da
• NCBI Official Full Name: carbonic anhydrase 9
• NCBI Official Synonym Full Names: carbonic anhydrase IX
• NCBI Official Symbol: CA9
• NCBI Official Synonym Symbols: MN; CAIX
• NCBI Protein Information: carbonic anhydrase 9
• UniProt Protein Name: Carbonic anhydrase 9
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA9
• UniProt Synonym Gene Names: G250; MN; CA-IX; CAIX; RCC-associated antigen G250
• UniProt Entry Name: CAH9_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

Uniprot Description

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: Nucleolus; Membrane protein, integral; Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: basolateral plasma membrane; integral to membrane; microvillus membrane; nucleolus; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to drug; response to testosterone stimulus; secretion