Human Neurogenic locus notch homolog protein 3 ELISA Kit | NOTCH3 elisa kit

Human Neurogenic locus notch homolog protein 3 ELISA Kit

Cat. Num. AAA762554

• Gene Names: NOTCH3; IMF2; LMNS; CASIL; CADASIL; CADASIL1
• Reactivity: Human
• Synonyms: Neurogenic locus notch homolog protein 3; Human Neurogenic locus notch homolog protein 3 ELISA Kit; NOTCH3/CADASIL/CASILneurogenic locus notch homolog protein 3/Notch (Drosophila) homolog 3/notch 3Notch homolog 3 (Drosophila)/Notch homolog 3; NOTCH3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NOTCH3. No significant cross-reactivity or interference between NOTCH3 and analogues was observed.
• Sequence Length: 2321

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for NOTCH3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 134244285
• NCBI GeneID: 4854
• NCBI Accession #: NP_000426.2
• NCBI GenBank Nucleotide #: NM_000435.2
• Molecular Weight: 243,631 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 3
• NCBI Official Synonym Full Names: notch 3
• NCBI Official Symbol: NOTCH3
• NCBI Official Synonym Symbols: IMF2; LMNS; CASIL; CADASIL; CADASIL1
• NCBI Protein Information: neurogenic locus notch homolog protein 3
• UniProt Protein Name: Neurogenic locus notch homolog protein 3
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: NOTCH3
• UniProt Synonym Gene Names: Notch 3
• UniProt Entry Name: NOTC3_HUMAN

NCBI Description

This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008]

Uniprot Description

NOTCH3: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH3. Interacts with PSMA1. Interacts with HIF1AN. Ubiquitously expressed in fetal and adult tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2-p13.1

Cellular Component: actin cytoskeleton; cytoplasm; cytosol; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral to membrane; nucleoplasm; plasma membrane; receptor complex

Molecular Function: calcium ion binding; enzyme binding; protein binding

Biological Process: artery morphogenesis; forebrain development; gene expression; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron fate commitment; Notch receptor processing; Notch signaling pathway; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

Disease: Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy; Lateral Meningocele Syndrome; Myofibromatosis, Infantile, 2