Mouse CD138 Monoclonal Antibody | anti-CD138 antibody

Anti-Hu CD138 PE

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Reactivity: Rat, Human, Non-human primates
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD138; Monoclonal Antibody; Anti-Hu CD138 PE; syndecan 1; SYND1; SDC; anti-CD138 antibody

• Host: Mouse
• Reactivity: Rat, Human, Non-human primates
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: MI15
• Specificity: The mouse monoclonal antibody MI15 recognizes an extracellular epitope of CD138 (syndecan 1), a 65-70kDa heparan sulfate proteoglycan expressed mainly in the epidermis and plasma cells, but also in growth factor-stimulated lymphocytes.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD138 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 10ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Conjugation: PE
• Immunogen: A mixture of U266 and XG-1 human myeloma cell lines
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD138 (MI15) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human peripheral blood mononucelar cells stained using anti-human CD138 (MI15) PE antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD38 (HIT2) APC antibody (10 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD138 antibody

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

References

*Nadalin MR, Fregnani ER, Silva-Sousa YT, Perez DE: Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions. Braz Dent J. 2011;22(3):223-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/21915520,*Jourdan M, Caraux A, Caron G, Robert N, Fiol G, Rème T, Bolloré K, Vendrell JP, Le Gallou S, Mourcin F, De Vos J, Kassambara A, Duperray C, Hose D, Fest T, Tarte K, Klein B: Characterization of a transitional preplasmablast population in the process of human B cell to plasma cell differentiation. J Immunol. 2011 Oct 15;187(8):3931-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/21918187,*Noll JE, Vandyke K, Hewett DR, Mrozik KM, Bala RJ, Williams SA, Kok CH, Zannettino AC: PTTG1 expression is associated with hyperproliferative disease and poor prognosis in multiple myeloma. J Hematol Oncol. 2015 Oct 6;8:106., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=vandyke+,*Atanackovic D, Hildebrandt Y, Templin J, Cao Y, Keller C, Panse J, Meyer S, Reinhard H, Bartels K, Lajmi N, Sezer O, Zander AR, Marx AH, Uhlig R, Zustin J, Bokemeyer C, Kröger N: Role of interleukin 16 in multiple myeloma. J Natl Cancer Inst. 2012 Jul 3;104(13):1005-20., URL: https://www.ncbi.nlm.nih.gov/pubmed/22745469,*Krishnan SR, Luk F, Brown RD, Suen H, Kwan Y, Bebawy M: Isolation of Human CD138(+) Microparticles from the Plasma of Patients with Multiple Myeloma. Neoplasia. 2016 Jan;18(1):25-32., URL: https://www.ncbi.nlm.nih.gov/pubmed/term=brown+rd,*Gattei V, Godeas C, Degan M, Rossi FM, Aldinucci D, Pinto A: Characterization of anti-CD138 monoclonal antibodies as tools for investigating the molecular polymorphism of syndecan-1 in human lymphoma cells. Br J Haematol. 1999 Jan;104(1):152-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/10027728
*Hayashida K, Johnston DR, Goldberger O, Park PW: Syndecan-1 expression in epithelial cells is induced by transforming growth factor beta through a PKA-dependent pathway. J Biol Chem. 2006 Aug 25;281(34):24365-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16807246ordin,*Choi DS, Kim JH, Ryu HS, Kim HC, Han JH, Lee JS, Min CK: Syndecan-1, a key regulator of cell viability in endometrial cancer. Int J Cancer. 2007 Aug 15;121(4):741-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/17455248ordin,*Manakil JF, Seymour GJ, Bartold PM: Effect of cytokine and antigen stimulation on peripheral blood lymphocyte syndecan-1 expression. Oral Microbiol Immunol. 2007 Aug;22(4):272-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17600540ordin,*Muto T, Miyoshi K, Munesue S, Nakada H, Okayama M, Matsuo T, Noma T: Differential expression of syndecan isoforms during mouse incisor amelogenesis. J Med Invest. 2007 Aug;54(3-4):331-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878683ordin,*Ojeh N, Hiilesvuo K, Wärri A, Salmivirta M, Henttinen T, Määttä A: Ectopic expression of syndecan-1 in basal epidermis affects keratinocyte proliferation and wound re-epithelialization. J Invest Dermatol. 2008 Jan;128(1):26-34., URL: http://www.ncbi.nlm.nih.gov/pubmed/17625591ordina,*Jilani I, Wei C, Bekele BN, Zhang ZJ, Keating M, Wierda W, Ferrajoli A, Estrov Z, Kantarjian H, O'Brien SM, Giles FJ, Albitar M: Soluble syndecan-1 (sCD138) as a prognostic factor independent of mutation status in patients with chronic lymphocytic leukemia. Int J Lab Hematol. 2008 Jan 7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18190591ordina

NCBI and Uniprot Product Information

• NCBI GI #: 55749480
• NCBI GeneID: 6382
• NCBI Accession #: NP_001006947.1
• NCBI GenBank Nucleotide #: NM_001006946.1
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523; AAB21434; AAB23935
• Molecular Weight: 32,462 Da
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor
• UniProt Protein Name: Syndecan-1
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1
• UniProt Entry Name: SDC1_HUMAN

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.

Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: lysosomal lumen; focal adhesion; cell surface; protein complex; Golgi lumen; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; response to cAMP; myoblast development; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; pathogenesis; lipoprotein metabolic process; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; odontogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; inflammatory response; response to calcium ion; retinoid metabolic process