Mouse Asialoglycoprotein Receptor 1 ELISA Kit | ASGR1 elisa kit

Mouse Asialoglycoprotein Receptor 1 ELISA Kit

Cat. Num. AAA762285

• Gene Names: ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1
• Reactivity: Mouse
• Synonyms: Asialoglycoprotein Receptor 1; Mouse Asialoglycoprotein Receptor 1 ELISA Kit; ASGR1/ASGPR1/CLEC4H1/MHL1/RHL1/ASGPR/ASGPR 1/ASGP-R 1/asialoglycoprotein receptor 1/CLEC4H1ASGPR1/C-type lectin domain family 4 member H1/Hepatic lectin H1/HL-1; ASGR1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ASGR1. No significant cross-reactivity or interference between ASGR1 and analogues was observed.
• Sequence Length: 291

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASGR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18149183
• NCBI GeneID: 432
• Molecular Weight: 29,144 Da
• NCBI Official Full Name: Asialoglycoprotein receptor 1
• NCBI Official Synonym Full Names: asialoglycoprotein receptor 1
• NCBI Official Symbol: ASGR1
• NCBI Official Synonym Symbols: HL-1; ASGPR; ASGPR1; CLEC4H1
• NCBI Protein Information: asialoglycoprotein receptor 1
• UniProt Protein Name: Asialoglycoprotein receptor 1
• Protein Family: Asialoglycoprotein receptor
• UniProt Gene Name: ASGR1
• UniProt Synonym Gene Names: CLEC4H1; ASGP-R 1; ASGPR 1; HL-1
• UniProt Entry Name: ASGR1_HUMAN

NCBI Description

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ASGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: extracellular region; integral to plasma membrane

Molecular Function: asialoglycoprotein receptor activity; carbohydrate binding; metal ion binding; protein binding; protein homodimerization activity

Biological Process: cellular response to extracellular stimulus; receptor-mediated endocytosis