Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CNTN3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CNTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CNTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CNTN3. You can calculate the concentration of CNTN3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
CNTN3: Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. Belongs to the immunoglobulin superfamily. Contactin family.
Protein type: Cell adhesion; Cell surface; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 3p12.3
Cellular Component: plasma membrane
Biological Process: nervous system development; cell adhesion