Rat Growth/differentiation factor 8 ELISA Kit | Mstn elisa kit

Rat Growth/differentiation factor 8 ELISA Kit

Cat. Num. AAA761998

• Gene Names: MSTN; GDF8; MSLHP
• Reactivity: Rat
• Synonyms: Growth/differentiation factor 8; Rat Growth/differentiation factor 8 ELISA Kit; Mstn/MSTN/Myostatin/GDF-8/GDF8growth differentiation factor 8/growth/differentiation factor 8/Myostatin/myostatin; Mstn elisa kit

• Reactivity: Rat
• Sequence Length: 375

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Mstn elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Mstn antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Mstn antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Mstn amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Mstn can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885259
• NCBI GeneID: 2660
• NCBI Accession #: NP_005250.1
• NCBI GenBank Nucleotide #: NM_005259.2
• Molecular Weight: 42,750 Da
• NCBI Official Full Name: growth/differentiation factor 8 preproprotein
• NCBI Official Synonym Full Names: myostatin
• NCBI Official Symbol: MSTN
• NCBI Official Synonym Symbols: GDF8; MSLHP
• NCBI Protein Information: growth/differentiation factor 8
• UniProt Protein Name: Growth/differentiation factor 8
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: MSTN
• UniProt Synonym Gene Names: GDF8; GDF-8
• UniProt Entry Name: GDF8_HUMAN

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Nov 2015]

Uniprot Description

MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; heparin binding; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell development; muscle development; muscle maintenance; negative regulation of insulin receptor signaling pathway; negative regulation of kinase activity; negative regulation of muscle hypertrophy; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of skeletal muscle growth; ovulation cycle process; positive regulation of defense response to virus by host; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade; response to electrical stimulus; response to estrogen stimulus; response to ethanol; response to gravity; response to heat; response to muscle activity; response to testosterone stimulus; skeletal muscle atrophy; skeletal muscle regeneration; transforming growth factor beta receptor signaling pathway

Disease: Muscle Hypertrophy