ATP6V1G3 recombinant protein

Recombinant Human ATP6V1G3

Cat. Num. AAA7614209

• Gene Names: ATP6V1G3; Vma10; ATP6G3
• Applications: Western Blot, ELISA
• Purity: Greater than 95% by SDS-PAGE gel analyses
• Synonyms: ATP6V1G3; Recombinant Human ATP6V1G3; ATP6G3; V ATPase 13kDa subunit 3; V ATPase subunit G 3; Vma10; ATP6V1G3 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95% by SDS-PAGE gel analyses
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
• Sequence Positions: 1-118

• Applicable Applications for ATP6V1G3 recombinant protein: Western Blot (WB), ELISA (EIA)
• Species: Human
• Tag: N-terminal His-Tag
• Reconstitution: Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
• Preparation and Storage: Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution

NCBI and Uniprot Product Information

• NCBI GI #: 72533514
• NCBI GeneID: 127124
• UniProt Accession #: Q96LB4; Q495K2; Q495K4; Q5T9L6
• Molecular Weight: 13,917 Da
• NCBI Official Full Name: ATP6V1G3 protein
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
• NCBI Official Symbol: ATP6V1G3
• NCBI Official Synonym Symbols: Vma10; ATP6G3
• NCBI Protein Information: V-type proton ATPase subunit G 3; V-ATPase G3 subunit; V-ATPase G subunit 3; V-ATPase subunit G 3; V-ATPase 13 kDa subunit 3; vacuolar proton pump G subunit 3; vacuolar proton pump subunit G 3; vacuolar proton pump, subunit G3; vacuolar ATP synthase subun
• UniProt Protein Name: V-type proton ATPase subunit G 3
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1G3
• UniProt Synonym Gene Names: ATP6G3; V-ATPase subunit G 3
• UniProt Entry Name: VATG3_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Subunit structure: V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d)

By similarity.

Tissue specificity: Kidney. Ref.1

Sequence similarities: Belongs to the V-ATPase G subunit family.