Human WBP2 (WW domain-binding protein 2) ELISA Kit | WBP2 elisa kit

Human WBP2 (WW domain-binding protein 2) ELISA Kit

Cat. Num. AAA7612211

• Gene Names: WBP2; WBP-2
• Reactivity: Human
• Synonyms: WBP2 (WW domain-binding protein 2); Human WBP2 (WW domain-binding protein 2) ELISA Kit; WW domain-binding protein 2; WBP2; WBP-2; WBP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WBP2. No significant cross-reactivity or interference between WBP2 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WBP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 24430132
• NCBI GeneID: 23558
• NCBI Accession #: NP_036610.2
• NCBI GenBank Nucleotide #: NM_012478.3
• UniProt Accession #: Q969T9; O95638
• Molecular Weight: 28,087 Da
• NCBI Official Full Name: WW domain-binding protein 2
• NCBI Official Synonym Full Names: WW domain binding protein 2
• NCBI Official Symbol: WBP2
• NCBI Official Synonym Symbols: WBP-2
• NCBI Protein Information: WW domain-binding protein 2
• UniProt Protein Name: WW domain-binding protein 2
• Protein Family: WW domain-binding protein
• UniProt Gene Name: WBP2
• UniProt Synonym Gene Names: WBP-2
• UniProt Entry Name: WBP2_HUMAN

NCBI Description

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein, which binds to the WW domain of Yes kinase-associated protein by its PY motifs. The function of this protein has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

WBP2: Binds to the WW domain of YAP1, WWP1 and WWP2. Interacts with NEDD4.

Protein type: Nuclear receptor co-regulator; Cell development/differentiation

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nuclear chromatin

Molecular Function: protein binding; chromatin DNA binding

Biological Process: establishment of protein localization; positive regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase II promoter