Human PEF1 (Peflin) ELISA Kit | PEF1 elisa kit

Human PEF1 (Peflin) ELISA Kit

Cat. Num. AAA7612168

• Gene Names: PEF1; ABP32; PEF1A
• Reactivity: Human
• Synonyms: PEF1 (Peflin); Human PEF1 (Peflin) ELISA Kit; Peflin; PEF protein with a long N-terminal hydrophobic domain; Penta-EF hand domain-containing protein 1; ABP32; UNQ1845; PRO3573; PEF1; PEF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PEF1. No significant cross-reactivity or interference between PEF1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PEF1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6912582
• NCBI GeneID: 553115
• NCBI Accession #: NP_036524.1
• NCBI GenBank Nucleotide #: NM_012392.3
• UniProt Accession #: Q9UBV8
• Molecular Weight: 30,381 Da
• NCBI Official Full Name: peflin
• NCBI Official Synonym Full Names: penta-EF-hand domain containing 1
• NCBI Official Symbol: PEF1
• NCBI Official Synonym Symbols: ABP32; PEF1A
• NCBI Protein Information: peflin; PEF protein with a long N-terminal hydrophobic domain; penta-EF hand domain containing 1
• UniProt Protein Name: Peflin
• Protein Family: Peflin
• UniProt Gene Name: PEF1
• UniProt Synonym Gene Names: ABP32
• UniProt Entry Name: PEF1_HUMAN

NCBI Description

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. The encoded protein has been shown to form a heterodimer with the programmed cell death 6 gene product and may modulate its function in Ca(2+) signaling. Alternative splicing results in multiple transcript variants and a pseudogene has been identified on chromosome 1.[provided by RefSeq, May 2010]

Uniprot Description

PEF1: Heterodimer; heterodimerizes with PDCD6/ALG2. Dissociates from PDCD6/ALG2 in presence of Ca(2+).

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: membrane; cytoplasm

Molecular Function: protein dimerization activity; protein binding; protein heterodimerization activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding

Biological Process: proteolysis; response to calcium ion