Rat Pulmonary Surfatcant Associated Protein B ELISA Kit | SPB elisa kit

Rat Pulmonary Surfatcant Associated Protein B ELISA Kit

Cat. Num. AAA761106

• Gene Names: SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• Reactivity: Rat
• Synonyms: Pulmonary Surfatcant Associated Protein B; Rat Pulmonary Surfatcant Associated Protein B ELISA Kit; SPB (Pulmonary Surfactant Associated Protein B)/SFTPB/SP-B/SFTP3/Pulmonary surfactant-associated proteolipid SPL (Phe)/18 kDa pulmonary-surfactant protein/6 kDa protein; SPB elisa kit

• Reactivity: Rat
• Sequence Length: 393

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SPB elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat SPB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat SPB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat SPB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat SPB can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 288856299
• NCBI GeneID: 6439
• NCBI Accession #: NP_000533.3
• NCBI GenBank Nucleotide #: NM_000542.3
• Molecular Weight: 42,117 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Synonym Full Names: surfactant protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• NCBI Protein Information: pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_HUMAN

NCBI Description

This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

SFTPB: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1); also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid- Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPB are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS). RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. A variation Ile to Thr at position 131 influences the association between specific alleles of SFTPA1 and respiratory distress syndrome in premature infants.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p12-p11.2

Cellular Component: clathrin-coated endocytic vesicle; endoplasmic reticulum membrane; extracellular region; extracellular space; lamellar body; lysosome; multivesicular body; nucleoplasm

Molecular Function: enzyme activator activity; G-protein-coupled receptor binding

Biological Process: cellular protein metabolic process; organ morphogenesis; positive regulation of catalytic activity; respiratory gaseous exchange; sphingolipid metabolic process

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 1