Rat Cytochrome P450 1B1 ELISA Kit | Cyp1b1 elisa kit

Rat Cytochrome P450 1B1 ELISA Kit

Cat. Num. AAA761099

• Gene Names: CYP1B1; CP1B; GLC3A; CYPIB1; P4501B1
• Reactivity: Rat
• Synonyms: Cytochrome P450 1B1; Rat Cytochrome P450 1B1 ELISA Kit; Cyp1b1/Cytochrome P450; family 1; subfamily B; polypeptide 1/Cytochrome P450RAP; Cyp1b1 elisa kit

• Reactivity: Rat
• Sequence Length: 543

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cyp1b1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Cyp1b1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Cyp1b1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Cyp1b1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Cyp1b1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 189491763
• NCBI GeneID: 1545
• NCBI Accession #: NP_000095.2
• NCBI GenBank Nucleotide #: NM_000104.3
• Molecular Weight: 60,846 Da
• NCBI Official Full Name: cytochrome P450 1B1
• NCBI Official Synonym Full Names: cytochrome P450 family 1 subfamily B member 1
• NCBI Official Symbol: CYP1B1
• NCBI Official Synonym Symbols: CP1B; GLC3A; CYPIB1; P4501B1
• NCBI Protein Information: cytochrome P450 1B1
• UniProt Protein Name: Cytochrome P450 1B1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1B1
• UniProt Entry Name: CP1B1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP1B1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A). GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG). POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes. Defects in CYP1B1 are a cause of Peters anomaly (PAN). Peters anomaly is a congenital defect of the anterior chamber of the eye. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.1; Oxidoreductase; Amino Acid Metabolism - tryptophan; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 2p22.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; mitochondrion

Molecular Function: heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: angiogenesis; arachidonic acid metabolic process; aromatic compound metabolic process; blood vessel morphogenesis; cell adhesion; collagen fibril organization; endothelial cell migration; epoxygenase P450 pathway; estrogen metabolic process; induction of apoptosis by oxidative stress; inhibition of NF-kappaB transcription factor; membrane lipid catabolic process; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of cell proliferation; nitric oxide biosynthetic process; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of JAK-STAT cascade; response to toxin; retinal metabolic process; retinol metabolic process; steroid metabolic process; sterol metabolic process; toxin metabolic process; visual perception; xenobiotic metabolic process

Disease: Glaucoma 3, Primary Congenital, A; Glaucoma 3, Primary Infantile, B; Peters Anomaly