Human Polycystin-2 ELISA Kit | PKD2 elisa kit

Human Polycystin-2 ELISA Kit

Cat. Num. AAA761096

• Gene Names: PKD2; PC2; PKD4; Pc-2; APKD2; TRPP2
• Reactivity: Human
• Synonyms: Polycystin-2; Human Polycystin-2 ELISA Kit; PKD2/Polycystin-2/Polycystwin/Autosomal dominant polycystic kidney disease type II protein/Polycystic kidney disease 2 protein; PKD2 elisa kit

• Reactivity: Human
• Sequence Length: 968

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PKD2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PKD2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PKD2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PKD2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PKD2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4505835
• NCBI GeneID: 5311
• NCBI Accession #: NP_000288.1
• NCBI GenBank Nucleotide #: NM_000297.3
• Molecular Weight: 98,838 Da
• NCBI Official Full Name: polycystin-2
• NCBI Official Synonym Full Names: polycystin 2, transient receptor potential cation channel
• NCBI Official Symbol: PKD2
• NCBI Official Synonym Symbols: PC2; PKD4; Pc-2; APKD2; TRPP2
• NCBI Protein Information: polycystin-2
• UniProt Protein Name: Polycystin-2
• Protein Family: Polycystin
• UniProt Gene Name: PKD2
• UniProt Entry Name: PKD2_HUMAN

NCBI Description

This gene encodes a member of the polycystin protein family. The encoded protein is a multi-pass membrane protein that functions as a calcium permeable cation channel, and is involved in calcium transport and calcium signaling in renal epithelial cells. This protein interacts with polycystin 1, and they may be partners in a common signaling cascade involved in tubular morphogenesis. Mutations in this gene are associated with autosomal dominant polycystic kidney disease type 2. [provided by RefSeq, Mar 2011]

Uniprot Description

PKD2: Involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Functions as a calcium permeable cation channel. Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2). ADPKD2 is a disorder characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. It represents approximately 15% of the cases of autosomal dominant polycystic kidney disease. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy. Belongs to the polycystin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, cation

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: basal cortex; basal plasma membrane; cilium; cytoplasm; cytosol; endoplasmic reticulum; endoplasmic reticulum membrane; filamentous actin; integral to plasma membrane; intercellular junction; lamellipodium; motile primary cilium; nonmotile primary cilium; plasma membrane

Molecular Function: actinin binding; ATPase binding; calcium ion binding; calcium-induced calcium release activity; cytoskeletal protein binding; HLH domain binding; identical protein binding; muscle alpha-actinin binding; phosphoprotein binding; potassium channel activity; protein binding; protein homodimerization activity; receptor binding; voltage-gated calcium channel activity; voltage-gated cation channel activity; voltage-gated ion channel activity; voltage-gated sodium channel activity

Biological Process: calcium ion transport; cell cycle arrest; centrosome duplication; cytoplasmic sequestering of transcription factor; detection of mechanical stimulus; determination of left/right symmetry; embryonic placenta development; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; heart development; heart looping; JAK-STAT cascade; liver development; negative regulation of cell proliferation; neural tube development; positive regulation of inositol-1,4,5-triphosphate receptor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; regulation of cAMP metabolic process; regulation of cell proliferation; release of sequestered calcium ion into cytosol; spinal cord development; ureteric bud branching

Disease: Polycystic Kidney Disease 2