Human Integrin alpha-4 ELISA Kit | ITGA4 elisa kit

Human Integrin alpha-4 ELISA Kit

Cat. Num. AAA761081

• Gene Names: ITGA4; IA4; CD49D
• Reactivity: Human
• Synonyms: Integrin alpha-4; Human Integrin alpha-4 ELISA Kit; ITGA4/Integrin alpha 4/CD49d/VLA-4/269C wild type/CD49 antigen-like family member D/CD49d antigen/IA4/integrin alpha-4/integrin alpha-4 subunit/Integrin alpha-IV/very late activation protein 4 receptor; alpha 4 subunit/VLA-4 subunit alpha; ITGA4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITG alphaVbeta3. No significant cross-reactivity or interference between ITG alphaVbeta3 and analogues was observed.
• Sequence Length: 1032

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.2-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITG alphaVbeta3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITG alphaVbeta3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGA4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ITG alpha Vbeta3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ITG alphaVbeta 3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITG alphaVbeta3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITG alphaV beta3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 67191027
• NCBI GeneID: 3676
• NCBI Accession #: NP_000876.3
• NCBI GenBank Nucleotide #: NM_000885.5
• Molecular Weight: 21,536 Da
• NCBI Official Full Name: integrin alpha-4 isoform 1 preproprotein
• NCBI Official Synonym Full Names: integrin subunit alpha 4
• NCBI Official Symbol: ITGA4
• NCBI Official Synonym Symbols: IA4; CD49D
• NCBI Protein Information: integrin alpha-4
• UniProt Protein Name: Integrin alpha-4
• Protein Family: Integrin
• UniProt Gene Name: ITGA4
• UniProt Synonym Gene Names: CD49D
• UniProt Entry Name: ITA4_HUMAN

NCBI Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

ITGA4: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Belongs to the integrin alpha chain family.

Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.3

Cellular Component: cell surface; external side of plasma membrane; focal adhesion; intercellular junction; membrane; plasma membrane

Molecular Function: cell adhesion molecule binding; fibronectin binding; metal ion binding; protein binding

Biological Process: B cell differentiation; blood coagulation; blood vessel remodeling; cell-matrix adhesion; diapedesis; extracellular matrix organization and biogenesis; heart development; heterophilic cell adhesion; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; receptor clustering; regulation of immune response