Human FOXO4 (Forkhead box protein O4) ELISA Kit | FOXO4 elisa kit

Human FOXO4 (Forkhead box protein O4) ELISA Kit

Cat. Num. AAA7608219

• Gene Names: FOXO4; AFX; AFX1; MLLT7
• Reactivity: Human
• Synonyms: FOXO4 (Forkhead box protein O4); Human FOXO4 (Forkhead box protein O4) ELISA Kit; Forkhead box protein O4; Fork head domain transcription factor AFX1; FOXO4; AFX; AFX1; MLLT7; FOXO4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FOXO4. No significant cross-reactivity or interference between FOXO4 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXO4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 283436083
• NCBI GeneID: 4303
• NCBI Accession #: NP_001164402.1
• NCBI GenBank Nucleotide #: NM_001170931.1
• UniProt Accession #: P98177; O43821; Q13720; Q3KPF1; Q8TDK9; B7WPJ7
• Molecular Weight: 53,684 Da
• NCBI Official Full Name: forkhead box protein O4 isoform 2
• NCBI Official Synonym Full Names: forkhead box O4
• NCBI Official Symbol: FOXO4
• NCBI Official Synonym Symbols: AFX; AFX1; MLLT7
• NCBI Protein Information: forkhead box protein O4; fork head domain transcription factor AFX1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
• UniProt Protein Name: Forkhead box protein O4
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO4
• UniProt Synonym Gene Names: AFX; AFX1; MLLT7
• UniProt Entry Name: FOXO4_HUMAN

NCBI Description

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

FOXO4: transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X;11)(q13;q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: epidermal growth factor receptor signaling pathway; transcription from RNA polymerase II promoter; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of smooth muscle cell differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; mitotic cell cycle G2/M transition DNA damage checkpoint; insulin receptor signaling pathway; innate immune response; cell cycle arrest