Human ANLN (Actin-binding protein anillin) ELISA Kit | ANLN elisa kit

Human ANLN (Actin-binding protein anillin) ELISA Kit

Cat. Num. AAA7608156

• Gene Names: ANLN; scra; Scraps
• Reactivity: Human
• Synonyms: ANLN (Actin-binding protein anillin); Human ANLN (Actin-binding protein anillin) ELISA Kit; Anillin; ANLN; ANLN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ANLN. No significant cross-reactivity or interference between ANLN and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANLN elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 546231404
• NCBI GeneID: 54443
• NCBI Accession #: NP_001271231.1
• NCBI GenBank Nucleotide #: NM_001284302.1
• UniProt Accession #: Q9NQW6; Q5CZ78; Q6NSK5; Q9H8Y4; Q9NVN9; Q9NVP0
• Molecular Weight: 124,199 Da
• NCBI Official Full Name: actin-binding protein anillin isoform 3
• NCBI Official Synonym Full Names: anillin, actin binding protein
• NCBI Official Symbol: ANLN
• NCBI Official Synonym Symbols: scra; Scraps
• NCBI Protein Information: actin-binding protein anillin
• UniProt Protein Name: Actin-binding protein anillin
• Protein Family: Anillin
• UniProt Gene Name: ANLN
• UniProt Entry Name: ANLN_HUMAN

NCBI Description

This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

anillin: an actin binding protein that interacts with cleavage furrow proteins such as septins and may play a role in cytokinesis.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 7p15-p14

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; contractile ring; actin cytoskeleton

Molecular Function: actin binding

Biological Process: mitosis; cytokinesis after mitosis; septin ring assembly; regulation of exit from mitosis; hemopoietic progenitor cell differentiation

Disease: Focal Segmental Glomerulosclerosis 8