Human FOXA1 (Forkhead box protein A1) ELISA Kit | FOXA1 elisa kit

Human FOXA1 (Forkhead box protein A1) ELISA Kit

Cat. Num. AAA7607069

• Gene Names: FOXA1; HNF3A; TCF3A
• Reactivity: Human
• Synonyms: FOXA1 (Forkhead box protein A1); Human FOXA1 (Forkhead box protein A1) ELISA Kit; FOXA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection ofFOXA1. No significant cross-reactivity or interference betweenFOXA1and analogues was observed.
• Sequence Length: 472

• Samples: serum, plasma, tissue homogenatesand other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelFOXA1were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelFOXA1were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXA1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FOXA1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FOXA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FOXA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FOXA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119586248
• NCBI GeneID: 3169
• Molecular Weight: 45,468 Da
• NCBI Official Full Name: forkhead box A1
• NCBI Official Synonym Full Names: forkhead box A1
• NCBI Official Symbol: FOXA1
• NCBI Official Synonym Symbols: HNF3A; TCF3A
• NCBI Protein Information: hepatocyte nuclear factor 3-alpha
• UniProt Protein Name: Hepatocyte nuclear factor 3-alpha
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXA1
• UniProt Synonym Gene Names: HNF3A; TCF3A; HNF-3-alpha; HNF-3A; TCF-3A

NCBI Description

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.