Human C-type lectin domain family 4 member E ELISA Kit | CLEC4E elisa kit

Human C-type lectin domain family 4 member E ELISA Kit

Cat. Num. AAA760254

• Gene Names: CLEC4E; MINCLE; CLECSF9
• Reactivity: Human
• Synonyms: C-type lectin domain family 4 member E; Human C-type lectin domain family 4 member E ELISA Kit; CLEC4E/C-type lectin domain family 4 member E/C-type lectin superfamily member 9/Macrophage-inducible C-type lectin/MINCLE/CLECSF9; CLEC4E elisa kit

• Reactivity: Human
• Sequence Length: 219

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CLEC4E elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CLEC4E antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CLEC4E antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CLEC4E amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CLEC4E can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7657333
• NCBI GeneID: 26253
• NCBI Accession #: NP_055173.1
• NCBI GenBank Nucleotide #: NM_014358.3
• Molecular Weight: 25,073 Da
• NCBI Official Full Name: C-type lectin domain family 4 member E
• NCBI Official Synonym Full Names: C-type lectin domain family 4 member E
• NCBI Official Symbol: CLEC4E
• NCBI Official Synonym Symbols: MINCLE; CLECSF9
• NCBI Protein Information: C-type lectin domain family 4 member E
• UniProt Protein Name: C-type lectin domain family 4 member E
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC4E
• UniProt Synonym Gene Names: CLECSF9; MINCLE
• UniProt Entry Name: CLC4E_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC4E: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbohydrate binding; receptor activity

Biological Process: defense response to bacterium; innate immune response; positive regulation of cytokine secretion; stimulatory C-type lectin receptor signaling pathway; T cell differentiation during immune response