Human Zona pellucida sperm-binding protein 3 ELISA Kit | ZP3 elisa kit

Human Zona pellucida sperm-binding protein 3 ELISA Kit

Cat. Num. AAA760091

• Gene Names: ZP3; ZPC; ZP3A; ZP3B; Zp-3
• Reactivity: Human
• Synonyms: Zona pellucida sperm-binding protein 3; Human Zona pellucida sperm-binding protein 3 ELISA Kit; ZP3 (Zona pellucida sperm-binding protein 3)/ZP3A/ZP3B/ZPC/Zp-3/Zona pellucida protein C/Zona pellucida glycoprotein 3/Sperm receptor/ZP3A/ZP3B/; ZP3 elisa kit

• Reactivity: Human
• Sequence Length: 424

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ZP3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ZP3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ZP3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ZP3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ZP3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 160358850
• NCBI GeneID: 7784
• NCBI Accession #: NP_001103824.1
• NCBI GenBank Nucleotide #: NM_001110354.1
• Molecular Weight: 41,272 Da
• NCBI Official Full Name: zona pellucida sperm-binding protein 3 isoform 1
• NCBI Official Synonym Full Names: zona pellucida glycoprotein 3 (sperm receptor)
• NCBI Official Symbol: ZP3
• NCBI Official Synonym Symbols: ZPC; ZP3A; ZP3B; Zp-3
• NCBI Protein Information: zona pellucida sperm-binding protein 3
• UniProt Protein Name: Zona pellucida sperm-binding protein 3
• Protein Family: Zona pellucida sperm-binding protein
• UniProt Gene Name: ZP3
• UniProt Synonym Gene Names: ZP3A; ZP3B; ZPC; Zp-3
• UniProt Entry Name: ZP3_HUMAN

NCBI Description

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZP3: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation. Belongs to the ZP domain family. ZPC subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Extracellular matrix

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: acrosome; cytoplasm; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; integral to membrane; multivesicular body; perinuclear region of cytoplasm; plasma membrane; proteinaceous extracellular matrix; secretory granule

Molecular Function: acrosin binding; carbohydrate binding; manganese ion transmembrane transporter activity; protein binding; signal transducer activity; store-operated calcium channel activity

Biological Process: binding of sperm to zona pellucida; blastocyst formation; humoral immune response mediated by circulating immunoglobulin; intracellular protein transport; manganese ion transport; multicellular organism reproduction; negative regulation of transcription, DNA-dependent; oocyte development; phosphoinositide-mediated signaling; positive regulation of humoral immune response; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-4 production; positive regulation of leukocyte migration; positive regulation of phosphatidylinositol biosynthetic process; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription, DNA-dependent; positive regulation of type IV hypersensitivity; single fertilization