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Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7600621 (IRF2BP2 Antibody) at dilution of 1:50)

Rabbit anti-Human, Mouse IRF2BP2 Polyclonal Antibody | anti-IRF2BP2 antibody

IRF2BP2 Rabbit Polyclonal

Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Synonyms
IRF2BP2; Polyclonal Antibody; IRF2BP2 Rabbit Polyclonal; IRF 2 binding protein 2; IRF 2BP2; anti-IRF2BP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Please decide the specificity by homology
Purity/Purification
Purification: Immunogen affinity purified
Purity: > = 95% as determined by SDS-PAGE
Form/Format
Liquid
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
571
Applicable Applications for anti-IRF2BP2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:4000
IP: 1:500-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
Immunogen
Interferon regulatory factor 2 binding protein 2
Preparation and Storage
Store at -20°C. (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7600621 (IRF2BP2 Antibody) at dilution of 1:50)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS7600621 (IRF2BP2 Antibody) at dilution of 1:50)

Immunofluorescence (IF)

(Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using MBS7600621 (IRF2BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using MBS7600621 (IRF2BP2 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L))

Immunoprecipitation (IP)

(IP Result of anti-IRF2BP2 (IP: MBS7600621, 3ug; Detection:1 MBS7600621 1:500) with K-562 cells lysate 2400ug.)

Immunoprecipitation (IP) (IP Result of anti-IRF2BP2 (IP: MBS7600621, 3ug; Detection:1 MBS7600621 1:500) with K-562 cells lysate 2400ug.)

SDS-PAGE

(Jurkat cells were subjected to SDS-PAGE followed by western blot with MBS7600621 (IRF2BP2 antibody) at dilution of 1:500)

SDS-PAGE (Jurkat cells were subjected to SDS-PAGE followed by western blot with MBS7600621 (IRF2BP2 antibody) at dilution of 1:500)
Related Product Information for anti-IRF2BP2 antibody
Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFATresponsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61 kDa
NCBI Official Full Name
interferon regulatory factor 2-binding protein 2 isoform B
NCBI Official Synonym Full Names
interferon regulatory factor 2 binding protein 2
NCBI Official Symbol
IRF2BP2
NCBI Protein Information
interferon regulatory factor 2-binding protein 2
UniProt Protein Name
Interferon regulatory factor 2-binding protein 2
UniProt Gene Name
IRF2BP2
UniProt Synonym Gene Names
IRF-2-binding protein 2; IRF-2BP2
UniProt Entry Name
I2BP2_HUMAN

NCBI Description

This gene encodes an interferon regulatory factor-2 (IRF2) binding protein that interacts with the C-terminal transcriptional repression domain of IRF2. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF2BP2: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle. Belongs to the IRF2BP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: cytoplasm; nucleus

Molecular Function: metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent

Research Articles on IRF2BP2

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Product Notes

The IRF2BP2 irf2bp2 (Catalog #AAA7600621) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRF2BP2 Rabbit Polyclonal reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IRF2BP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:1000-1:4000 IP: 1:500-1:1000 IHC: 1:20-1:200 IF: 1:20-1:200. Researchers should empirically determine the suitability of the IRF2BP2 irf2bp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRF2BP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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