Human Apelin ELISA Kit

Human Total Apelin (Apelin) Elisa kit

Cat. Num. AAA756419

• Reactivity: Human
• Synonyms: Apelin; Human Total Apelin (Apelin) Elisa kit; Apelin elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apelin. No significant cross-reactivity or interference between Apelin and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Apelin and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Apelin elisa kit

Intended Uses: This Apelin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Apelin. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: Apelin ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Apelin antibody and an Apelin-HRP conjugate. The assay sample and buffer are incubated together with Apelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apelin concentration since Apelin from samples and Apelin-HRP conjugate compete for the anti-Apelin antibody binding site. Since the number of sites is limited, as more sites are occupied by Apelin from the sample, fewer sites are left to bind Apelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apelin concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6708146
• Protein Family: Apelin

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: apelin receptor binding; G-protein-coupled receptor binding; hormone activity; receptor binding

Biological Process: drinking behavior; immune response; lactation; negative regulation of blood pressure; negative regulation of vasoconstriction; positive regulation of adrenocorticotropic hormone secretion; positive regulation of cell proliferation; positive regulation of corticotropin-releasing hormone secretion; positive regulation of heat generation; positive regulation of phosphorylation; positive regulation of vasodilation; regulation of respiratory gaseous exchange; regulation of the force of heart contraction; signal transduction