Porcine GATA Binding Protein 4 ELISA Kit | GATA4 elisa kit

Porcine GATA Binding Protein 4 ELISA Kit

Cat. Num. AAA755622

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Reactivity: Porcine
• Synonyms: GATA Binding Protein 4; Porcine GATA Binding Protein 4 ELISA Kit; GATA4 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GATA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GATA4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATA4 elisa kit

Principle of the assay: GATA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GATA4 antibody and an GATA4-HRP conjugate. The assay sample and buffer are incubated together with GATA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GATA4 concentration since GATA4 from samples and GATA4-HRP conjugate compete for the anti-GATA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by GATA4 from the sample, fewer sites are left to bind GATA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GATA4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GATA4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 219518812
• NCBI GeneID: 2626
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: GATA binding protein 4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4; GATA-binding factor 4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq, Jul 2008]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; DNA binding; transcription activator binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; negative regulation of autophagy; positive regulation of transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; cell-cell signaling; regulation of transcription, DNA-dependent; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to mechanical stimulus; response to estrogen stimulus; endoderm formation; positive regulation of cardioblast differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; endoderm development

Disease: Ventricular Septal Defect 1; Atrial Septal Defect 2; Tetralogy Of Fallot; Testicular Anomalies With Or Without Congenital Heart Disease; Atrioventricular Septal Defect 4