Rat Lin28 ELISA Kit | Lin28 elisa kit

Rat Lin28 ELISA Kit

Cat. Num. AAA754175

• Gene Names: LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• Reactivity: Rat
• Synonyms: Lin28; Rat Lin28 ELISA Kit; Lin28 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Preparation and Storage: Store all reagents at 2-8 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 20306208
• NCBI GeneID: 79727
• Molecular Weight: 22,743 Da
• NCBI Official Full Name: LIN28 protein
• NCBI Official Synonym Full Names: lin-28 homolog A (C. elegans)
• NCBI Official Symbol: LIN28A
• NCBI Official Synonym Symbols: CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• NCBI Protein Information: protein lin-28 homolog A; RNA-binding protein LIN-28; zinc finger, CCHC domain containing 1; zinc finger CCHC domain-containing protein 1
• UniProt Protein Name: Protein lin-28 homolog A
• UniProt Gene Name: LIN28A
• UniProt Synonym Gene Names: CSDD1; LIN28; ZCCHC1; Lin-28A
• UniProt Entry Name: LN28A_HUMAN

Uniprot Description

LIN28A: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family.

Protein type: Nucleolus; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: stress granule; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; miRNA binding; DNA binding; zinc ion binding; RNA binding; translation initiation factor binding

Biological Process: regulation of transcription, DNA-dependent; positive regulation of translation; somatic stem cell maintenance; pre-microRNA processing; stem cell maintenance; positive regulation of neuron differentiation; RNA 3'-end processing; germ cell development; negative regulation of glial cell differentiation