Typical Testing Data/Standard Curve (for reference only)
Rabbit Beta-Defensin 14 ELISA Kit | DEFb14 elisa kit
Rabbit Beta-Defensin 14 ELISA Kit
Gene Names
Defb14; BD-14
Reactivity
Rabbit
Synonyms
Beta-Defensin 14; Rabbit Beta-Defensin 14 ELISA Kit; DEFb14 elisa kit
Reactivity
Rabbit
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This DEFb14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit DEFb14. This ELISA kit for research use only, not for therapeutic applications
!Intended Uses
This DEFb14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit DEFb14. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DEFb14 elisa kit
Principle of the Assay: DEFb14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFb14 antibody and an DEFb14-HRP conjugate. The assay sample and buffer are incubated together with DEFb14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFb14 concentration since DEFb14 from samples and DEFb14-HRP conjugate compete for the anti-DEFb14 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFb14 from the sample, fewer sites are left to bind DEFb14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFb14 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DEFb14 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,718 Da
NCBI Official Full Name
Defb14 protein
NCBI Official Synonym Full Names
defensin beta 14
NCBI Official Symbol
Defb14
NCBI Official Synonym Symbols
BD-14
NCBI Protein Information
beta-defensin 14
UniProt Protein Name
Beta-defensin 14
Protein Family
UniProt Gene Name
Defb14
UniProt Synonym Gene Names
BD-14; mBD-14
UniProt Entry Name
DFB14_MOUSE
Uniprot Description
Has antibacterial activity.
Research Articles on DEFb14
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Product Notes
The Rabbit DEFb14 defb14 (Catalog #AAA752511) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752511 ELISA Kit recognizes Rabbit DEFb14. It is sometimes possible for the material contained within the vial of "Beta-Defensin 14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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