Typical Testing Data/Standard Curve (for reference only)
Rat Neural Cell Adhesion Molecule ELISA Kit | NCAM elisa kit
Rat Neural Cell Adhesion Molecule ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NCAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NCAM antibody and an NCAM-HRP conjugate. The assay sample and buffer are incubated together with NCAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NCAM concentration since NCAM from samples and NCAM-HRP conjugate compete for the anti-NCAM antibody binding site. Since the number of sites is limited, as more sites are occupied by NCAM from the sample, fewer sites are left to bind NCAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NCAM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Uniprot Description
NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: identical protein binding
Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion
Research Articles on NCAM
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Product Notes
The Rat NCAM ncam1 (Catalog #AAA752333) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752333 ELISA Kit recognizes Rat NCAM. It is sometimes possible for the material contained within the vial of "Neural Cell Adhesion Molecule, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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