Typical Testing Data/Standard Curve (for reference only)
Bovine Heat Shock 70kDa Protein 4 ELISA Kit | HSPA4 elisa kit
Bovine Heat Shock 70kDa Protein 4 ELISA Kit
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Reactivity
Bovine
Synonyms
Heat Shock 70kDa Protein 4; Bovine Heat Shock 70kDa Protein 4 ELISA Kit; HSPA4 elisa kit
Reactivity
Bovine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSPA4 elisa kit
Principle of the assay: HSPA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSPA4 antibody and an HSPA4-HRP conjugate. The assay sample and buffer are incubated together with HSPA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSPA4 concentration since HSPA4 from samples and HSPA4-HRP conjugate compete for the anti-HSPA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSPA4 from the sample, fewer sites are left to bind HSPA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSPA4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HSPA4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,331 Da
NCBI Official Full Name
heat shock 70 kDa protein 4
NCBI Official Synonym Full Names
heat shock 70kDa protein 4
NCBI Official Symbol
HSPA4
NCBI Official Synonym Symbols
RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
NCBI Protein Information
heat shock 70 kDa protein 4; hsp70 RY; heat shock 70kD protein 4; heat shock protein, 110 kDa; heat shock 70-related protein APG-2; epididymis secretory sperm binding protein Li 5a
UniProt Protein Name
Heat shock 70 kDa protein 4
Protein Family
UniProt Gene Name
HSPA4
UniProt Synonym Gene Names
APG2
UniProt Entry Name
HSP74_HUMAN
Uniprot Description
HSP70RY: Interacts with TJP1/ZO-1. Belongs to the heat shock protein 70 family.
Protein type: Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: mitochondrion; cytosol
Molecular Function: ATP binding
Biological Process: chaperone-mediated protein complex assembly; protein import into mitochondrial outer membrane; response to unfolded protein
Research Articles on HSPA4
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Product Notes
The Bovine HSPA4 hspa4 (Catalog #AAA751949) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA751949 ELISA Kit recognizes Bovine HSPA4. It is sometimes possible for the material contained within the vial of "Heat Shock 70kDa Protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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