Human Sigma non opioid intracellular receptor 1 ELISA Kit | SIGMAR1 elisa kit

Human Sigma non opioid intracellular receptor 1 ELISA Kit

Cat. Num. AAA751929

• Gene Names: Sigmar1; Oprs1; sigma1R; AL024364
• Reactivity: Human
• Synonyms: Sigma non opioid intracellular receptor 1; Human Sigma non opioid intracellular receptor 1 ELISA Kit; SIGMAR1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGMAR1 elisa kit

Principle of the assay: SIGMAR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIGMAR1 antibody and an SIGMAR1-HRP conjugate. The assay sample and buffer are incubated together with SIGMAR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIGMAR1 concentration since SIGMAR1 from samples and SIGMAR1-HRP conjugate compete for the anti-SIGMAR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SIGMAR1 from the sample, fewer sites are left to bind SIGMAR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIGMAR1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SIGMAR1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6754942
• NCBI GeneID: 18391
• NCBI Accession #: NP_035144.1
• NCBI GenBank Nucleotide #: NM_011014.3
• Molecular Weight: 21,633 Da
• NCBI Official Full Name: sigma non-opioid intracellular receptor 1 isoform 1
• NCBI Official Synonym Full Names: sigma non-opioid intracellular receptor 1
• NCBI Official Symbol: Sigmar1
• NCBI Official Synonym Symbols: Oprs1; sigma1R; AL024364
• NCBI Protein Information: sigma non-opioid intracellular receptor 1; sigma1-receptor; opioid receptor, sigma 1; sigma 1-type opioid receptor; sigma 1 receptor variant SR-1D
• UniProt Protein Name: Sigma non-opioid intracellular receptor 1
• Protein Family: Sigma non-opioid intracellular receptor
• UniProt Gene Name: Sigmar1
• UniProt Synonym Gene Names: Oprs1; Sigma1-receptor; Sigma1R
• UniProt Entry Name: SGMR1_MOUSE

NCBI Description

This gene encodes a transmembrane protein located in the endoplasmic reticulum. The encoded protein is a receptor that binds several endogenous ligands, including N,N-dimethyltryptamine, progesterone and pregnenolone and a variety of of non-opiate compounds. The encoded protein plays a role in regulating the activity of ion channels, acting as a chaperone and protecting cells from oxidative stress. In humans, this receptor has been associated with Alzheimer's and Parkinson's diseases, stroke and numerous disease conditions such as depression, pain and addiction. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2013]

Uniprot Description

SIGMAR1: Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. Defects in SIGMAR1 are the cause of amyotrophic lateral sclerosis type 16, juvenile (ALS16). ALS16 is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5- 10% of the cases. Belongs to the ERG2 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Nuclear envelope; Receptor, misc.

Cellular Component: cell projection; membrane; endoplasmic reticulum; plasma membrane; integral to membrane; nuclear envelope; lipid particle; cell junction; nucleus

Molecular Function: protein binding; opioid receptor activity; receptor activity

Biological Process: nervous system development; transport; regulation of neuron apoptosis; lipid transport