Monkey Adenylate Cyclase 4 ELISA Kit | ADCY4 elisa kit

Monkey Adenylate Cyclase 4 ELISA Kit

Cat. Num. AAA751924

• Gene Names: ADCY4; AC4
• Reactivity: Monkey
• Synonyms: Adenylate Cyclase 4; Monkey Adenylate Cyclase 4 ELISA Kit; ADCY4 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCY4 elisa kit

Principle of the assay: ADCY4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADCY4 antibody and an ADCY4-HRP conjugate. The assay sample and buffer are incubated together with ADCY4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADCY4 concentration since ADCY4 from samples and ADCY4-HRP conjugate compete for the anti-ADCY4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADCY4 from the sample, fewer sites are left to bind ADCY4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADCY4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADCY4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 34785029
• NCBI GeneID: 196883
• Molecular Weight: 46,245 Da
• NCBI Official Full Name: ADCY4 protein, partial
• NCBI Official Synonym Full Names: adenylate cyclase 4
• NCBI Official Symbol: ADCY4
• NCBI Official Synonym Symbols: AC4
• NCBI Protein Information: adenylate cyclase type 4; adenylyl cyclase 4; ATP pyrophosphate-lyase 4; adenylate cyclase type IV
• UniProt Protein Name: Adenylate cyclase type 4
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY4
• UniProt Entry Name: ADCY4_HUMAN

NCBI Description

This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]

Uniprot Description

ADCY4: This is a membrane-bound, calmodulin-insensitive adenylyl cyclase. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Nucleotide Metabolism - purine; Membrane protein, multi-pass; EC 4.6.1.1; Membrane protein, integral; Transporter, aquaporin family; Lyase; Transporter; Adenylyl cyclase

Chromosomal Location of Human Ortholog: 14q12

Cellular Component: membrane; dendrite; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; metal ion binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport