Sheep Angiotensinase A ELISA Kit | ATA elisa kit

Sheep Angiotensinase A ELISA Kit

Cat. Num. AAA749422

• Reactivity: Sheep
• Synonyms: Angiotensinase A; Sheep Angiotensinase A ELISA Kit; ATA elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ATA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ATA. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATA elisa kit

Principle of the Assay: ATA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATA antibody and an ATA-HRP conjugate. The assay sample and buffer are incubated together with ATA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATA concentration since ATA from samples and ATA-HRP conjugate compete for the anti-ATA antibody binding site. Since the number of sites is limited, as more sites are occupied by ATA from the sample, fewer sites are left to bind ATA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATA elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 7159085
• NCBI GeneID: 64017
• Molecular Weight: 64,959 Da
• NCBI Official Full Name: aminopeptidase A
• NCBI Official Synonym Full Names: glutamyl aminopeptidase
• NCBI Official Symbol: Enpep
• NCBI Protein Information: glutamyl aminopeptidase; EAP; AP-A; aminopeptidase A
• UniProt Protein Name: Glutamyl aminopeptidase
• Protein Family: Adhesin Ata autotransporter
• UniProt Gene Name: Enpep
• UniProt Synonym Gene Names: EAP; AP-A
• UniProt Entry Name: AMPE_RAT

NCBI Description

ectoenzyme that selectively hydrolyzes acidic amino acid residues from the amino terminus of oligopeptides [RGD, Feb 2006]

Uniprot Description

ENPEP: Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. Belongs to the peptidase M1 family.

Protein type: EC 3.4.11.7; Protease; Membrane protein, integral

Cellular Component: apical part of cell; lysosomal membrane; apical plasma membrane; integral to membrane; vesicle membrane; cytoplasmic vesicle; brush border; external side of plasma membrane

Molecular Function: peptidase activity; zinc ion binding; metallopeptidase activity; aminopeptidase activity; peptide binding

Biological Process: cell proliferation; cell migration; aromatic compound metabolic process; angiogenesis; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; glomerulus development