Typical Testing Data/Standard Curve (for reference only)
Monkey A Disintegrin And Metalloprotease 9 ELISA Kit | ADAM9 elisa kit
Monkey A Disintegrin And Metalloprotease 9 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADAM9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAM9 antibody and an ADAM9-HRP conjugate. The assay sample and buffer are incubated together with ADAM9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM9 concentration since ADAM9 from samples and ADAM9-HRP conjugate compete for the anti-ADAM9 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM9 from the sample, fewer sites are left to bind ADAM9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM9 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Protease; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8p11.22
Cellular Component: extracellular space; focal adhesion; cell surface; basolateral plasma membrane; cytoplasm; integral to membrane
Molecular Function: collagen binding; integrin binding; protein binding; protein kinase C binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; laminin binding; SH3 domain binding
Biological Process: integrin-mediated signaling pathway; positive regulation of keratinocyte migration; extracellular matrix organization and biogenesis; activation of MAPKK activity; cell-matrix adhesion; membrane protein ectodomain proteolysis; response to glucocorticoid stimulus; positive regulation of cell adhesion mediated by integrin; positive regulation of membrane protein ectodomain proteolysis; keratinocyte differentiation; response to manganese ion; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; response to hydrogen peroxide; positive regulation of protein secretion; transforming growth factor beta receptor signaling pathway; cell-cell adhesion mediated by integrin; cell adhesion mediated by integrin; response to calcium ion; cell adhesion
Disease: Cone-rod Dystrophy 9
Research Articles on ADAM9
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Product Notes
The Monkey ADAM9 adam9 (Catalog #AAA747945) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA747945 ELISA Kit recognizes Monkey ADAM9. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 9, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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