Related Product Information for Ig-Hc elisa kit
Principle of the Assay: Ig-Hc ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Ig-Hc. Standards or samples are then added to the microtiter plate wells and Ig-Hc if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Ig-Hc present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Ig-Hc are added to each well to "sandwich" the Ig-Hc immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Ig-Hc and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ig-Hc concentration in each sample is interpolated from this standard curve.
Product Categories/Family for Ig-Hc elisa kit
Product Notes
The Chicken Ig-Hc (Catalog #
AAA746870) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746870 ELISA Kit recognizes Chicken Ig-Hc.
It is sometimes possible for the material contained within the vial of
"Heavy Chain Immunoglobulin, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
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responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
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