Bovine Collagenase, Type II ELISA Kit | Collagenase II elisa kit

Bovine Collagenase, Type II ELISA Kit

Cat. Num. AAA746413

• Reactivity: Bovine
• Synonyms: Collagenase; Type II; Bovine Collagenase; Type II ELISA Kit; Collagenase II elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL-2. No significant cross-reactivity or interference between COL-2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COL-2 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Collagenase II elisa kit

Intended Uses: This COL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine COL-2. This ELISA kit for research use only!

Principle of the Assay: COL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COL-2 antibody and an COL-2-HRP conjugate. The assay sample and buffer are incubated together with COL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL-2 concentration since COL-2 from samples and COL-2-HRP conjugate compete for the anti-COL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL-2 from the sample, fewer sites are left to bind COL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL-2 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 203499
• NCBI GeneID: 171052
• Molecular Weight: 53,375 Da
• NCBI Official Full Name: collagenase, partial
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: Mmp13
• NCBI Protein Information: collagenase 3; MMP-13; UMRCASE; collagenase-3; matrix metalloproteinase 13; matrix metalloproteinase-13
• UniProt Protein Name: Collagenase 3
• UniProt Gene Name: Mmp13
• UniProt Synonym Gene Names: MMP-13
• UniProt Entry Name: MMP13_RAT

NCBI Description

enzyme that degrades collagen type I [RGD, Feb 2006]

Uniprot Description

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().