Sheep Contactin Associated Protein 1 ELISA Kit | CNTNAP1 elisa kit

Sheep Contactin Associated Protein 1 ELISA Kit

Cat. Num. AAA745755

• Gene Names: CNTNAP1; P190; CASPR; NRXN4; CNTNAP
• Reactivity: Sheep
• Synonyms: Contactin Associated Protein 1; Sheep Contactin Associated Protein 1 ELISA Kit; CNTNAP1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CNTNAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CNTNAP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNTNAP1 elisa kit

Principle of the Assay: CNTNAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CNTNAP1 antibody and an CNTNAP1-HRP conjugate. The assay sample and buffer are incubated together with CNTNAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNTNAP1 concentration since CNTNAP1 from samples and CNTNAP1-HRP conjugate compete for the anti-CNTNAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNTNAP1 from the sample, fewer sites are left to bind CNTNAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNTNAP1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4505463
• NCBI GeneID: 8506
• NCBI Accession #: NP_003623.1
• NCBI GenBank Nucleotide #: NM_003632.2
• Molecular Weight: 156,267 Da
• NCBI Official Full Name: contactin-associated protein 1
• NCBI Official Synonym Full Names: contactin associated protein 1
• NCBI Official Symbol: CNTNAP1
• NCBI Official Synonym Symbols: P190; CASPR; NRXN4; CNTNAP
• NCBI Protein Information: contactin-associated protein 1; caspr1; neurexin 4; neurexin-4; neurexin IV
• UniProt Protein Name: Contactin-associated protein 1
• Protein Family: Contactin-associated protein
• UniProt Gene Name: CNTNAP1
• UniProt Synonym Gene Names: CASPR; NRXN4; Caspr; Caspr1
• UniProt Entry Name: CNTP1_HUMAN

NCBI Description

The gene product was initially identified as a 190-kD protein associated with the contactin-PTPRZ1 complex. The 1,384-amino acid protein, also designated p190 or CASPR for 'contactin-associated protein,' includes an extracellular domain with several putative protein-protein interaction domains, a putative transmembrane domain, and a 74-amino acid cytoplasmic domain. Northern blot analysis showed that the gene is transcribed predominantly in brain as a transcript of 6.2 kb, with weak expression in several other tissues tested. The architecture of its extracellular domain is similar to that of neurexins, and this protein may be the signaling subunit of contactin, enabling recruitment and activation of intracellular signaling pathways in neurons. [provided by RefSeq, Jan 2009]

Uniprot Description

CNTNAP1: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells. Belongs to the neurexin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: voltage-gated potassium channel complex; integral to plasma membrane; paranode region of axon; integral to membrane

Molecular Function: protein binding; SH3/SH2 adaptor activity; receptor activity; SH3 domain binding

Biological Process: neuromuscular process controlling posture; axon guidance; positive regulation of signal transduction; action potential propagation; axon cargo transport; cytoskeleton organization and biogenesis; cell adhesion; neuromuscular process controlling balance; signal transduction; neurite morphogenesis; paranodal junction assembly

Disease: Lethal Congenital Contracture Syndrome 7