Monkey Hydroxyacylglutathione hydrolase, Mitochondrial ELISA Kit | HAGH elisa kit

Monkey Hydroxyacylglutathione hydrolase, Mitochondrial ELISA Kit

Cat. Num. AAA745350

• Gene Names: HAGH; GLO2; GLX2; GLXII; HAGH1
• Reactivity: Monkey
• Synonyms: Hydroxyacylglutathione hydrolase; Mitochondrial; Monkey Hydroxyacylglutathione hydrolase; Mitochondrial ELISA Kit; HAGH elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This HHM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey HHM. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HAGH elisa kit

Principle of the assay: HHM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HHM antibody and an HHM-HRP conjugate. The assay sample and buffer are incubated together with HHM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HHM concentration since HHM from samples and HHM-HRP conjugate compete for the anti-HHM antibody binding site. Since the number of sites is limited, as more sites are occupied by HHM from the sample, fewer sites are left to bind HHM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HHM concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94538322
• NCBI GeneID: 3029
• NCBI Accession #: NP_005317.2
• NCBI GenBank Nucleotide #: NM_005326.4
• Molecular Weight: 25,455 Da
• NCBI Official Full Name: hydroxyacylglutathione hydrolase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: hydroxyacylglutathione hydrolase
• NCBI Official Symbol: HAGH
• NCBI Official Synonym Symbols: GLO2; GLX2; GLXII; HAGH1
• NCBI Protein Information: hydroxyacylglutathione hydrolase, mitochondrial; glx II; glyoxalase II; hydroxyacylglutathione hydroxylase
• UniProt Protein Name: Hydroxyacylglutathione hydrolase, mitochondrial
• UniProt Gene Name: HAGH
• UniProt Synonym Gene Names: GLO2; HAGH1; Glx II
• UniProt Entry Name: GLO2_HUMAN

NCBI Description

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

HAGH: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid. Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.6; Hydrolase; Mitochondrial; Carbohydrate Metabolism - pyruvate

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial matrix; cytoplasm

Molecular Function: hydroxyacylglutathione hydrolase activity; zinc ion binding

Biological Process: glutathione biosynthetic process

Disease: Hydroxyacyl Glutathione Hydrolase Deficiency