Typical Testing Data/Standard Curve (for reference only)
Canine Anti-Glycolipid AutoAntibody ELISA Kit | AGA elisa kit
Canine Anti-Glycolipid AutoAntibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AGA ELISA kit applies the competitive enzyme immunoassay technique utilizing Glycolipid antigen and an AGA-HRP conjugate. The assay sample and buffer are incubated together with AGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGA concentration since AGA from samples and AGA-HRP conjugate compete for the Glycolipid antigen binding site. Since the number of sites is limited, as more sites are occupied by AGA from the sample, fewer sites are left to bind AGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Jan 2010]
Uniprot Description
AGA: a catabolic enzyme, aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme.
Protein type: Hydrolase; Glycan Metabolism - other glycan degradation; EC 3.5.1.26
Chromosomal Location of Human Ortholog: 4q34.3
Cellular Component: lysosome; endoplasmic reticulum
Molecular Function: peptidase activity; protein self-association; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity
Biological Process: protein maturation; protein deglycosylation; proteolysis
Disease: Aspartylglucosaminuria
Research Articles on AGA
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Product Notes
The Canine AGA aga (Catalog #AAA744537) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA744537 ELISA Kit recognizes Canine AGA. It is sometimes possible for the material contained within the vial of "Anti-Glycolipid AutoAntibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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