Goat Dendritic cell Associated C type Lectin 1 ELISA Kit | DCAL-2 elisa kit

Goat Dendritic cell Associated C type Lectin 1 ELISA Kit

Cat. Num. AAA743384

• Gene Names: CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12
• Reactivity: Goat
• Synonyms: Dendritic cell Associated C type Lectin 1; Goat Dendritic cell Associated C type Lectin 1 ELISA Kit; DCAL-2 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This DCAL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat DCAL-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DCAL-2 elisa kit

Principle of the Assay: DCAL-2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DCAL-2. Standards or samples are then added to the microtiter plate wells and DCAL-2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DCAL-2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for DCAL-2 are added to each well to "sandwich" the DCAL-2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DCAL-2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCAL-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DCAL-2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 13649708
• NCBI GeneID: 64581
• Molecular Weight: 13,138 Da
• NCBI Official Full Name: dendritic cell-associated C-type lectin-1
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; lectin-like receptor 1; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12
• UniProt Protein Name: C-type lectin domain family 7 member A
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; UNQ539/PRO1082; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; plasma membrane; integral to membrane

Molecular Function: metal ion binding; carbohydrate binding; MHC protein binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; phagocytosis, recognition; cell recognition; carbohydrate mediated signaling; pattern recognition receptor signaling pathway; defense response to protozoan; inflammatory response

Disease: Aspergillosis, Susceptibility To; Candidiasis, Familial, 4