Typical Testing Data/Standard Curve (for reference only)
Bovine alpha1-Microglobulin ELISA Kit | alpha1-MG elisa kit
Bovine alpha1-Microglobulin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ?1-MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ?1-MG. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ?1-MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-?1-MG antibody and an ?1-MG-HRP conjugate. The assay sample and buffer are incubated together with ?1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity ofcolor is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ?1-MG concentration since ?1-MG from samples and ?1-MG-HRP conjugate compete for the anti-?1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by ?1-MG from the sample, fewer sites are left to bind ?1-MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ?1-MG concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]
Uniprot Description
AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q32-q33
Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; plasma membrane; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; calcium oxalate binding; heme binding; calcium channel inhibitor activity
Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage
Research Articles on alpha1-MG
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Product Notes
The Bovine alpha1-MG ambp (Catalog #AAA743116) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743116 ELISA Kit recognizes Bovine alpha1-MG. It is sometimes possible for the material contained within the vial of "alpha1-Microglobulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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