Sheep Motilin ELISA Kit | MTL elisa kit

Sheep Motilin ELISA Kit

Cat. Num. AAA742745

• Reactivity: Sheep
• Synonyms: Motilin; Sheep Motilin ELISA Kit; MTL elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This MTL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep MTL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MTL elisa kit

Principle of the Assay: MTL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MTL antibody and an MTL-HRP conjugate. The assay sample and buffer are incubated together with MTL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTL concentration since MTL from samples and MTL-HRP conjugate compete for the anti-MTL antibody binding site. Since the number of sites is limited, as more sites are occupied by MTL from the sample, fewer sites are left to bind MTL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTL concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 386955
• NCBI GeneID: 4295
• Molecular Weight: 12,849 Da
• NCBI Official Full Name: motilin
• NCBI Official Synonym Full Names: motilin
• NCBI Official Symbol: MLN
• NCBI Protein Information: promotilin; prepromotilin
• UniProt Protein Name: Promotilin
• Protein Family: Motilin
• UniProt Gene Name: MLN
• UniProt Synonym Gene Names: MAP
• UniProt Entry Name: MOTI_HUMAN

NCBI Description

This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene. [provided by RefSeq, May 2010]

Uniprot Description

MLN: Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle. Belongs to the motilin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell-cell signaling