Porcine Glucokinase ELISA Kit | GCK elisa kit

Porcine Glucokinase ELISA Kit

Cat. Num. AAA742414

• Gene Names: Gck; GLUKA; RNGK2
• Reactivity: Porcine
• Synonyms: Glucokinase; Porcine Glucokinase ELISA Kit; GCK elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCK elisa kit

Intended Uses: This GCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GCK. This ELISA kit for research use only!

Principle of the Assay: GCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCK antibody and an GCK-HRP conjugate. The assay sample and buffer are incubated together with GCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCK concentration since GCK from samples and GCK-HRP conjugate compete for the anti-GCK antibody binding site. Since the number of sites is limited, as more sites are occupied by GCK from the sample, fewer sites are left to bind GCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7110599
• NCBI GeneID: 24385
• NCBI Accession #: NP_036697.1
• NCBI GenBank Nucleotide #: NM_012565.2
• Molecular Weight: 51,919 Da
• NCBI Official Full Name: glucokinase isoform 2
• NCBI Official Synonym Full Names: glucokinase
• NCBI Official Symbol: Gck
• NCBI Official Synonym Symbols: GLUKA; RNGK2
• NCBI Protein Information: glucokinase; HK4; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; glucokinase variant 3
• UniProt Protein Name: Glucokinase
• Protein Family: Glucokinase
• UniProt Gene Name: Gck
• UniProt Synonym Gene Names: HK IV; HK4
• UniProt Entry Name: HXK4_RAT

NCBI Description

catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]

Uniprot Description

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.