Chicken Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit | sFLt-1 elisa kit

Chicken Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit

Cat. Num. AAA740803

• Gene Names: Flt1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• Reactivity: Chicken
• Synonyms: Soluble Fms-like tyrosine kinase Receptor 1; Chicken Soluble Fms-like tyrosine kinase Receptor 1 ELISA Kit; sFLt-1 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This sFLt-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken sFLt-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for sFLt-1 elisa kit

Principle of the Assay: sFLt-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sFLt-1 antibody and an sFLt-1-HRP conjugate. The assay sample and buffer are incubated together with sFLt-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sFLt-1 concentration since sFLt-1 from samples and sFLt-1-HRP conjugate compete for the anti-sFLt-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by sFLt-1 from the sample, fewer sites are left to bind sFLt-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sFLt-1 concentration in each sample is interpolated from this standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 2809071
• NCBI GeneID: 14254
• UniProt Accession #: O55095; P35969; Q3TJC1; Q5GRG7; Q78ZF4; Q8K1H4; Q9CSC8; Q9CW58; Q9DAV2
• Molecular Weight: 77,522 Da
• NCBI Official Full Name: sFlt-1
• NCBI Official Synonym Full Names: FMS-like tyrosine kinase 1
• NCBI Official Symbol: Flt1
• NCBI Official Synonym Symbols: Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757
• NCBI Protein Information: vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1
• UniProt Protein Name: SFlt-1
• UniProt Gene Name: Flt1
• UniProt Entry Name: O55095_MOUSE

Uniprot Description

VEGFR1: a receptor tyrosine kinase of the VEGFR family. Receptor for VEGF, VEGFB and PGF. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Two splice variant isoforms have been described. Isoform SFlt1 may have an inhibitory role in angiogenesis.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; TK group; VEGFR family

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; integral to membrane; endosome; receptor complex

Molecular Function: transferase activity; identical protein binding; vascular endothelial growth factor receptor activity; growth factor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; chemotaxis; protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; patterning of blood vessels; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; positive regulation of angiogenesis; positive regulation of MAPKKK cascade; response to hypoxia; blood vessel morphogenesis; sprouting angiogenesis; embryonic morphogenesis; angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration