Sheep TEK Tyrosine Kinase, Endothelial ELISA Kit | Tie2 elisa kit

Sheep TEK Tyrosine Kinase, Endothelial ELISA Kit

Cat. Num. AAA740733

• Reactivity: Sheep
• Synonyms: TEK Tyrosine Kinase; Endothelial; Sheep TEK Tyrosine Kinase; Endothelial ELISA Kit; Tie2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Tie2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Tie2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tie2 elisa kit

Principle of the Assay: Tie2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Tie2 antibody and an Tie2 -HRP conjugate. The assay sample and buffer are incubated together with Tie2 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Tie2 concentration since Tie2 from samples and Tie2 -HRP conjugate compete for the anti-Tie2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Tie2 from the sample, fewer sites are left to bind Tie2 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tie2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Tie2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 56462535
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 109,141 Da
• UniProt Protein Name: Angiopoietin-1 receptor
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Kinase, protein; Protein kinase, tyrosine (receptor); Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: microvillus; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; stress fiber

Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; cell-matrix adhesion; protein amino acid autophosphorylation; heart development; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; angiogenesis; endochondral ossification; organ regeneration; Tie receptor signaling pathway; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; regulation of vascular permeability; positive regulation of protein amino acid phosphorylation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal