Typical Testing Data/Standard Curve (for reference only)
Canine TEK Tyrosine Kinase, Endothelial ELISA Kit | Tie2 elisa kit
Canine TEK Tyrosine Kinase, Endothelial ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Tie2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Tie2 antibody and an Tie2-HRP conjugate. The assay sample and buffer are incubated together with Tie2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Tie2 concentration since Tie2 from samples and Tie2-HRP conjugate compete for the anti-Tie2 antibody binding site. Since the number of sites is limited, as more sites are occupied by Tie2 from the sample, fewer sites are left to bind Tie2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Tie2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family
Chromosomal Location of Human Ortholog: 9p21
Cellular Component: cell surface; focal adhesion; microvillus; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; basal plasma membrane; stress fiber
Molecular Function: protein binding; protein-tyrosine kinase activity; growth factor binding; receptor activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity
Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; protein amino acid autophosphorylation; cell-matrix adhesion; heart development; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; regulation of vascular permeability; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Disease: Venous Malformations, Multiple Cutaneous And Mucosal
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Product Notes
The Canine Tie2 tek (Catalog #AAA736430) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA736430 ELISA Kit recognizes Canine Tie2. It is sometimes possible for the material contained within the vial of "TEK Tyrosine Kinase, Endothelial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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