Canine Gamma Glutamyltransferase 1 ELISA Kit | gGT1 elisa kit

Canine Gamma Glutamyltransferase 1 ELISA Kit

Cat. Num. AAA739358

• Gene Names: GGT1; GGT; GTG; CD224; GGT 1; D22S672; D22S732
• Reactivity: Canine
• Synonyms: Gamma Glutamyltransferase 1; Canine Gamma Glutamyltransferase 1 ELISA Kit; gGT1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This gammaGT-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine gammaGT-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for gGT1 elisa kit

Principle of the Assay: gammaGT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gammaGT-1 antibody and an gammaGT-1-HRP conjugate. The assay sample and buffer are incubated together with gammaGT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gammaGT-1 concentration since gammaGT-1 from samples and gammaGT-1-HRP conjugate compete for the anti-gammaGT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by gammaGT-1 from the sample, fewer sites are left to bind gammaGT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gammaGT-1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47678519
• NCBI GeneID: 2678
• UniProt Accession #: P19440; Q08247; Q14404; Q8TBS1; Q9UMK1
• Molecular Weight: 24,080 Da
• NCBI Official Full Name: GGT1
• NCBI Official Synonym Full Names: gamma-glutamyltransferase 1
• NCBI Official Symbol: GGT1
• NCBI Official Synonym Symbols: GGT; GTG; CD224; GGT 1; D22S672; D22S732
• NCBI Protein Information: gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase
• UniProt Protein Name: Gamma-glutamyltranspeptidase 1
• Protein Family: Gamma-glutamyltranspeptidase
• UniProt Gene Name: GGT1
• UniProt Synonym Gene Names: GGT; GGT 1
• UniProt Entry Name: GGT1_HUMAN

NCBI Description

The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]

Uniprot Description

GGT1: Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Catalyzes the transfer of the glutamyl moiety of glutathione to amino acids and dipeptide acceptors. Alternatively, glutathione can be hydrolyzed to give Cys-Gly and gamma glutamate. Isoform 3 seems to be inactive. Defects in GGT1 are a cause of glutathionuria (GLUTH); also known as gamma-glutamyltranspeptidase deficiency. It is an autosomal recessive disease. Belongs to the gamma-glutamyltransferase family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.19.13; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - selenoamino acid; EC 2.3.2.2; Transferase; EC 3.4.19.14; Other Amino Acids Metabolism - cyanoamino acid; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: anchored to external side of plasma membrane; extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; gamma-glutamyltransferase activity

Biological Process: amino acid metabolic process; glutamate metabolic process; regulation of immune system process; cysteine biosynthetic process; leukotriene metabolic process; proteolysis; leukotriene biosynthetic process; glutathione metabolic process; xenobiotic metabolic process; regulation of inflammatory response; glutathione catabolic process; glutathione biosynthetic process; arachidonic acid metabolic process; spermatogenesis; zymogen activation

Disease: Glutathionuria