Goat Voltage-gated calcium channel ELISA Kit | VGCC elisa kit

Goat Voltage-gated calcium channel ELISA Kit

Cat. Num. AAA738533

• Gene Names: CACNA1C; TS; LQT8; CACH2; CACN2; CaV1.2; CCHL1A1; CACNL1A1
• Reactivity: Goat
• Synonyms: Voltage-gated calcium channel; Goat Voltage-gated calcium channel ELISA Kit; VGCC elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This VGCC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat VGCC. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VGCC elisa kit

Principle of the Assay: VGCC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for VGCC. Standards or samples are then added to the microtiter plate wells and VGCC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of VGCC present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for VGCC are added to each well to "sandwich" the VGCC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain VGCC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VGCC concentration in each sample is interpolated from this standard curve.

Product Categories/Family for VGCC elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 951207
• NCBI GeneID: 775
• UniProt Accession #: O95234; Q13936; Q4G0H8; Q59GU3; Q5V9X8; Q5V9X9; Q6YL47; Q86XX0; Q86XX1
• Molecular Weight: 4,122 Da
• NCBI Official Full Name: voltage-gated calcium channel, partial
• NCBI Official Synonym Full Names: calcium channel, voltage-dependent, L type, alpha 1C subunit
• NCBI Official Symbol: CACNA1C
• NCBI Official Synonym Symbols: TS; LQT8; CACH2; CACN2; CaV1.2; CCHL1A1; CACNL1A1
• NCBI Protein Information: voltage-dependent L-type calcium channel subunit alpha-1C; DHPR, alpha-1 subunit; calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit; calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10*
• UniProt Protein Name: Calcium channel L-type alpha 1 subunit
• UniProt Entry Name: O95234_HUMAN

NCBI Description

This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CACNA1C: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. Defects in CACNA1C are the cause of Timothy syndrome (TS). TS is a disorder characterized by multiorgan dysfunction including lethal arrhythmias, webbing of fingers and toes, congenital heart disease, immune deficiency, intermittent hypoglycemia, cognitive abnormalities and autism. Defects in CACNA1C are the cause of Brugada syndrome type 3 (BRGDA3). A heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. 35 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cell soma; cytoplasm; postsynaptic density; caveolar macromolecular signaling complex; integral to membrane; plasma membrane; voltage-gated calcium channel complex; dendritic shaft; Z disc

Molecular Function: voltage-gated calcium channel activity; calmodulin binding; protein binding; enzyme binding; metal ion binding; high voltage-gated calcium channel activity; alpha-actinin binding

Biological Process: axon guidance; synaptic transmission; growth hormone secretion; elevation of cytosolic calcium ion concentration; smooth muscle contraction involved in micturition; insulin secretion; regulation of blood pressure; regulation of vasoconstriction; energy reserve metabolic process; visual learning; regulation of organ growth; glucose homeostasis; regulation of insulin secretion; adult walking behavior; calcium ion-dependent exocytosis

Disease: Timothy Syndrome; Brugada Syndrome 3